goat anti notch3  (R&D Systems)

Journal: Genes & Diseases

Article Title: NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue

doi: 10.1016/j.gendis.2015.05.003

Figure Lengend Snippet: Detection of NOTCH3 and CD146 in human apical papilla. (A) Immunohistochemical staining of the apical papilla, showing NOTCH3 staining is associated with blood vessels. (B) Staining for NOTCH3, (C) control. (D–H) Immunoflourescence staining of the apical papilla showing blood vessels stained for D) NOTCH3 (green), (E) CD146 (red), (F) co-localization of NOTCH3 and CD146, (G) DAPI (blue), (H) co-localization of NOTCH3, CD146 and DAPI. Data are representative of independent experiments of samples from 2 donors each performed in triplicate.

Article Snippet: Cells were then incubated with goat polyclonal anti-NOTCH3 antibody (dilution 1:25, clone M-20, Santa Cruz Biotechnology Inc., USA) overnight at 4 °C.

Techniques: Immunohistochemical staining, Staining, Control

Journal: Genes & Diseases

Article Title: NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue

doi: 10.1016/j.gendis.2015.05.003

Figure Lengend Snippet: Expression of NOTCH3 and JAG1 in SCAP at passage 3. (A, B) Immunocytochemical staining of SCAP, showing positive staining for NOTCH3 in the perinuclear region (indicated by the white arrow in A) and in the nucleus (yellow arrow head in A). (C–H) Immunoflourescence staining of SCAP showing positive staining for NOTCH3 (green) and JAG1 (red). Note that NOTCH3 is located in the perinuclear region and in the nucleus while JAG1 only in the cytplasm and perinuclear region. In (C–E), isolated single cells showing both NOTCH3 and JAG1 in the same cell. Similar finding is in (F–H) when cells are in contact with each other. Data are representative of independent experiments of samples from 3 donors each performed in triplicate.

Article Snippet: Cells were then incubated with goat polyclonal anti-NOTCH3 antibody (dilution 1:25, clone M-20, Santa Cruz Biotechnology Inc., USA) overnight at 4 °C.

Techniques: Expressing, Staining, Isolation

Journal: Genes & Diseases

Article Title: NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue

doi: 10.1016/j.gendis.2015.05.003

Figure Lengend Snippet: Flow cytometry analysis of SCAP at passage 3. (A) Representative images of SCAP of ∼80% confluence before subjected to flow cytometry. (B) SCAP double staining with NOTCH3 and STRO-1: 7% is expressing NOTCH3, 15.8% expressing STRO-1 and 1.5% expressing both STRO-1 and NOTCH3. (C) Flow cytometry dot plots showing triple- staining of SCAP for CD146 (red), STRO-1 (purple) and NOTCH3 (blue). Note the triple positive population of cells are shown in green. Data are representative of 2 experiments performed in triplicate.

Article Snippet: Cells were then incubated with goat polyclonal anti-NOTCH3 antibody (dilution 1:25, clone M-20, Santa Cruz Biotechnology Inc., USA) overnight at 4 °C.

Techniques: Flow Cytometry, Double Staining, Expressing, Staining

Journal: Genes & Diseases

Article Title: NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue

doi: 10.1016/j.gendis.2015.05.003

Figure Lengend Snippet: Flow cytometry analysis of SCAP at passage 3 underwent osteogenesis. (A) Control SCAP expressing NOTCH3; (B) SCAP after osteogenic differentiation for 14 days losing NOTCH3 expression. Representative data from 2 experiments in triplicate.

Article Snippet: Cells were then incubated with goat polyclonal anti-NOTCH3 antibody (dilution 1:25, clone M-20, Santa Cruz Biotechnology Inc., USA) overnight at 4 °C.

Techniques: Flow Cytometry, Control, Expressing